C-SRC monoclonal antibody

• Background:

  The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells . Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active .

• Structure Composition:

  Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

• Swiss Prot:

  P12931

• Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant fusion protein of human c-SRC. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Related Pathways:

  Regulation of Actin Dynamics, Adherens Junction signaling, Tumor Angiogenesis, ErbB HER Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, Jak Stat : IL-6 Receptor Signaling, Inhibition of Apoptosis, Regulation of Microtubule Dynamics, Signaling Pathways Activating p38 MAP Kinase, Wntβ catenin signaling

• Applications:

  WB, IF/ICC

• Dilution:

  WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50)

• Purity:

  This antibody is purified through a protein G column.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 60 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of c-SRC protein.

• Applications Notes:

  Western blot analysis of c-SRC expression in HT29 (A) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4