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CDK4 monoclonal antibody

CAT: 0384-MB66868-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-MB66868-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control . Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions . Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins . Motif is found in critical regulatory proteins including Bad, cdc25C, FKHRL1, PKC and c-Raf . Phospho-14-3-3 Binding Motif Polyclonal and Monoclonal Antibodies provide powerful tools for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.
CAS Number
9007-83-4
Structure Composition
Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3
Swiss Prot
P11802
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein of human CDK4. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB, IHC
Dilution
WB (1/500 - 1/1000), IHC (1/50 - 1/200)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 30 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of CDK4 protein.
Applications Notes
Western blot analysis of CDK4 expression in A431 (A), Hela (B), K562 (C), MCF7 (D), NIH3T3 (E) whole cell lysates.
Host or Source
Mouse

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