Lambda light chain monoclonal antibody

• Background:

  V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V- (D) -J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.

• Structure Composition:

  Mouse IgG2a kappa. Liquid in PBS, and 0.01% sodium azide.

• Product Name Alternative:

  Immunoglobulin lambda variable 1-51; Ig lambda chain V-I region BL2; Ig lambda chain V-I region EPS; Ig lambda chain V-I region NEW; Ig lambda chain V-I region NIG-64

• Swiss Prot:

  P01701

• Reactivity:

  Human

• Immunogen:

  This antibody is generated from mouse immunized with Lambda light chain recombinant protein.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC

• Dilution:

  WB (1/500 - 1/1000), IHC (1/20 - 1/50)

• Purity:

  This antibody is purified through a protein G column.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 28 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of Lambda light chain protein.

• Applications Notes:

  Western blot analysis of Lambda light chain expression in Ramos (A) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4