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Lambda light chain monoclonal antibody

CAT: 0384-MB66706-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-MB66706-01Size:50 µL
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Background
V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V- (D) -J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
CAS Number
9007-83-4
Structure Composition
Mouse IgG2a kappa. Liquid in PBS, and 0.01% sodium azide.
Product Name Alternative
Immunoglobulin lambda variable 1-51; Ig lambda chain V-I region BL2; Ig lambda chain V-I region EPS; Ig lambda chain V-I region NEW; Ig lambda chain V-I region NIG-64
Swiss Prot
P01701
Reactivity
Human
Immunogen
This antibody is generated from mouse immunized with Lambda light chain recombinant protein.
Conjugation
Unconjugated
Applications
WB, IHC
Dilution
WB (1/500 - 1/1000), IHC (1/20 - 1/50)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 28 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of Lambda light chain protein.
Applications Notes
Western blot analysis of Lambda light chain expression in Ramos (A) whole cell lysates.
Host or Source
Mouse

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