Synaptophysin monoclonal antibody

• Background:

  Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms. Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore. Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases. Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  Synaptophysin; Major synaptic vesicle protein p38

• Swiss Prot:

  P08247

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Purified recombinant fragment of SYP expressed in E. Coli.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC

• Dilution:

  WB (1/500 - 1/1000), IHC (1/50 - 1/100)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 38 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of Synaptophysin protein.

• Applications Notes:

  Western blot analysis of Synaptophysin expression in rat brain (A), rat hippocampus (B), Hela (C), NIH3T3 (D) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4