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Synaptophysin monoclonal antibody

CAT: 0384-MB66637-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-MB66637-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms. Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore. Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases. Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
Synaptophysin; Major synaptic vesicle protein p38
Swiss Prot
P08247
Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of SYP expressed in E. Coli.
Conjugation
Unconjugated
Applications
WB, IHC
Dilution
WB (1/500 - 1/1000), IHC (1/50 - 1/100)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 38 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of Synaptophysin protein.
Applications Notes
Western blot analysis of Synaptophysin expression in rat brain (A), rat hippocampus (B), Hela (C), NIH3T3 (D) whole cell lysates.
Host or Source
Mouse

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