NF-kappaB p105 monoclonal antibody

• Background:

  Transcription factors of the nuclear factor κB (NF-κB) /Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52) . Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression. NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus. Following IKK-mediated phosphorylation of p105 NF-κB at multiple sites (Ser921, 923, 927, and 932) on its carboxy-terminus, SCF/β-TrCP mediated processing produces the 50 kDa active form p50.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

• Swiss Prot:

  P19838

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant human NF-κB1 p105/p50 protein.

• Conjugation:

  Unconjugated

• Related Pathways:

  Regulation of Apoptosis, Death Receptor Signaling, Inhibition of Apoptosis, NF-kB Signaling

• Applications:

  WB

• Dilution:

  WB (1/500 - 1/1000)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 105 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of NF-kappaB p105 protein.

• Applications Notes:

  Western blot analysis of NF-kappaB p105 expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4