EPO Receptor polyclonal antibody

• Background:

  Erythropoiesis is regulated through the interaction of erythropoietin (Epo) with its receptor, EpoR, a member of the cytokine superfamily of receptors. The human EpoR is a 507 amino acid transmembrane protein that forms homo_x0002_dimers following erythropoietin activation and is related to the interleukin 2 (IL-2) receptor b-chain subunit (IL-2Rb) . EpoR and IL-2Rb share 45% amino acid identity within the box 1 and box 2 domains of their cytoplasmic regions while their remaining cytoplasmic sequences are highly divergent. These conserved domains are both required and sufficient for mitogenesis and for coupling ligand binding to the induction of tyrosine phosphorylation. The membrane proximal region is also required for the association of JAK2 with EpoR. The existence of multiple cross-linked complexes and differential ligand affinities suggests that EpoR may exist as a multireceptor complex.

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  Erythropoietin receptor; EPO-R

• Swiss Prot:

  P19235

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein corresponding to human EPO Receptor. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, IF/ICC

• Dilution:

  WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 52 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of EPO Receptor protein.

• Applications Notes:

  Western blot analysis of EPO Receptor expression in K562 (A), mouse kidney (B), rat liver (C) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4