Products for Research Use Only

IL-1 beta polyclonal antibody

CAT: 0384-BS67752-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS67752-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses. It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form. Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin
Swiss Prot
P01584
Reactivity
Human, Mouse, Rat
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IL-1 beta. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB, IHC, IF/ICC, IP
Dilution
WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200), IP (1/50 - 1/100)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 16 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of IL-1 beta protein.
Applications Notes
Western blot analysis of IL-1 beta expression in A549 (A), Raw264.7 (B), mouse liver (C), rat kidney (D) whole cell lysates.
Host or Source
Rabbit