PARP2 polyclonal antibody

• Background:

  Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair . Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units . Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage . Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1 . Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site . PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks . HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP2 in order to limit the length of poly-ADP-ribose chains . Specifically mediates formation of branched poly-ADP-ribosylation . Branched poly-ADP-ribose chains are specifically recognized by some factors, such as APLF . In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex .

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  ADPRT2; ADPRTL2; Poly [ADP-ribose] polymerase 2; PARP-2; hPARP-2; ADP-ribosyltransferase diphtheria toxin-like 2; ARTD2; NAD (+) ADP-ribosyltransferase 2; ADPRT-2; Poly[ADP-ribose] synthase 2; pADPRT-2

• Swiss Prot:

  Q9UGN5

• Reactivity:

  Human, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PARP2. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB (1/500 - 1/1000)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 62 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of PARP2 protein.

• Applications Notes:

  Western blot analysis of PARP2 expression in HEK293T (A), MCF7 (B), rat brain (C) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4