Vinculin polyclonal antibody

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development . Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail . In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration . Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-α and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction . This change in vinculin allows the binding of a number of other proteins, including talin, α-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state. Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth . This is supported by a demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma.

9007-83-4

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Vinculin; Metavinculin; MV

P18206

Human, Mouse, Rat, Chicken, Pig

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Vinculin. The exact sequence is proprietary.

Unconjugated

WB

WB (1/500 - 1/1000)

The antibody was purified by immunogen affinity chromatography.

Unmodification

~ 130 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

Recognizes endogenous levels of Vinculin protein.

Western blot analysis of Vinculin expression in Hela (A), HepG2 (B), PC12 (C), mouse muscle (D) whole cell lysates.