DARPP32 polyclonal antibody

• Background:

  DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum. It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity . Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 . DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) . Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype.

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein

• Swiss Prot:

  Q9UD71

• Reactivity:

  Human, Mouse, Rat, Pig

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DARPP32. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Related Pathways:

  Dopamine Signaling in Parkinson\'s Disease Plathway.

• Applications:

  WB, IHC

• Dilution:

  WB (1/500 - 1/1000), IHC (1/50 - 1/200)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 37 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of DARPP32 protein.

• Applications Notes:

  Western blot analysis of DARPP32 expression in mouse heart (A), rat heart (B) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4