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SCXA polyclonal antibody

CAT: 0384-BS66161-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS66161-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Basic helix loop helix transcription factor scleraxis; Basic helix-loop-helix transcription factor scleraxis; bHLHa41; bHLHa48; Class A basic helix-loop-helix protein 41; Class A basic helix-loop-helix protein 48; scleraxis homolog A; SCX; SCX_HUMAN; SCXB
Swiss Prot
Q7RTU7
Reactivity
Human, Mouse, Rat
Immunogen
KLH conjugated synthetic peptide derived from human SCXA : 131-201/201
Conjugation
Unconjugated
Applications
ELISA IHC IF ICC
Dilution
ELISA: 1:5000-1:10000 IHC: 1:100-1:500 IF: 1:100-1:500 ICC: 1:100-1:500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 22 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
SCXA polyclonal antibody detects endogenous levels of SCXA protein.
Applications Notes
Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-SCXA Polyclonal Antibody, Unconjugated (bs-12364R) 1:200, overnight at 4°C.
Host or Source
Rabbit

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