SCXA polyclonal antibody

• Background:

  Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Basic helix loop helix transcription factor scleraxis; Basic helix-loop-helix transcription factor scleraxis; bHLHa41; bHLHa48; Class A basic helix-loop-helix protein 41; Class A basic helix-loop-helix protein 48; scleraxis homolog A; SCX; SCX_HUMAN; SCXB

• Swiss Prot:

  Q7RTU7

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH conjugated synthetic peptide derived from human SCXA : 131-201/201

• Conjugation:

  Unconjugated

• Applications:

  ELISA IHC IF ICC

• Dilution:

  ELISA: 1:5000-1:10000 IHC: 1:100-1:500 IF: 1:100-1:500 ICC: 1:100-1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 22 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  SCXA polyclonal antibody detects endogenous levels of SCXA protein.

• Applications Notes:

  Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-SCXA Polyclonal Antibody, Unconjugated (bs-12364R) 1:200, overnight at 4°C.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4