AMPKα1 polyclonal antibody

• Background :

  The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK) . AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

• Structure Composition :

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  PRKAA1; AMPK; AMPKa1; protein kinase AMP-activated catalytic subunit alpha 1

• Swiss Prot :

  Q13131

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A synthetic peptide of human AMPKα1 (NP_006242.5) .

• Conjugation :

  Unconjugated

• Applications :

  IF/ICC

• Dilution :

  IF/ICC,1:50 - 1:20

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  Refer to figures

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  Unmodification

• Applications Notes :

  Immunofluorescence analysis of HeLa cells using AMPKα1 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4