TFEB polyclonal antibody

• Background:

  Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'; efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  TFEB; ALPHATFEB; BHLHE35; TCFEB

• Swiss Prot:

  P34897

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human TFEB (NP_009093.1) .

• Conjugation:

  Unconjugated

• Applications:

  WB, IF/ICC, IHC

• Dilution:

  WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IHC,1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  Refer to figures

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Unmodification

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4