PDHA1 polyclonal antibody

• Background :

  The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) . The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• Structure Composition :

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  PDHA1; PDHA; PDHAD; PDHCE1A; PHE1A

• Swiss Prot :

  P08559

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant fusion protein of human PDHA1 (NP_000275.1) .

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC, IF/ICC, IP

• Dilution :

  WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,1:20 - 1:50

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  43KDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  Unmodification

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4