Phospho-Cyclin D1-T286 polyclonal antibody

• Background:

  The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  BCL1; D11S287E; PRAD1; U21B31; CCND1; Cyclin D1; cyclin D1

• Swiss Prot:

  P24385

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic phosphorylated peptide around T286 of human Cyclin D1 (NP_444284.1) .

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC

• Dilution:

  WB,1:500 - 1:2000|IHC,1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylated

• Molecular Weight:

  37kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Phosphorylated

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4