SUMO4 polyclonal antibody

• Background :

  This gene is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene, which leads to the M55V substitution, has been associated with type I diabetes. The RefSeq contains this polymorphism.

• Structure Composition :

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  SUMO4; IDDM5; SMT3H4; SUMO-4; dJ281H8.4

• Swiss Prot :

  Q6EEV6

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant fusion protein of human SUMO4 (NP_001002255.1) .

• Conjugation :

  Unconjugated

• Applications :

  WB, IF/ICC

• Dilution :

  WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  15kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  Unmodification

• Applications Notes :

  Western blot analysis of extracts of various cell lines, using SUMO4 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 40s.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4