Phospho-CFL1-Y139 polyclonal antibody

• Background:

  The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  CFL1; CFL; HEL-S-15; cofilin; cofilin-1

• Swiss Prot:

  P23528

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic phosphorylated peptide around Y139 of human Phospho-CFL1-Y139 (NP_005498.1) .

• Conjugation:

  Unconjugated

• Applications:

  IHC

• Dilution:

  IHC,1:50 - 1:100

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylated

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Phosphorylated

• Applications Notes:

  Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-CFL1-Y139 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4