Pan-Tri-Methyl lysine polyclonal antibody

• Background:

  Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs) .The post-translational ε-amino lysine methylated proteins is an important reversible modification which plays a vital role in the regulation of many cellular processes including chromatin dynamics and gene transcription. Methylation of lysine residues is modulated by specific counteractive enzymes including lysine methylases (KMTs) and demethylases (KDMs) . Lysine trimethylation occurs in both histones and non-histone substratres. It has become promising targets for discovery of anti-cancer drugs.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Reactivity:

  Human, Mouse, Rat, Other (Wide Range)

• Immunogen:

  A synthetic peptide corresponding to a sequence containing Tri-methylatedK.

• Conjugation:

  Unconjugated

• Applications:

  WB, IP, ChIP

• Dilution:

  WB,1:500 - 1:2000|IP,1:50 - 1:200|ChIP,1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Methylated

• Molecular Weight:

  15-74KDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Methylated

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4