Chk2 polyclonal antibody

• Background:

  These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function.

• Structure Composition:

  Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Product Name Alternative:

  CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2

• Swiss Prot:

  O96017

• Reactivity:

  Human

• Immunogen:

  A synthesized peptide derived from human Chk2

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC-P, ICC/IF, IP

• Dilution:

  WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

• Purity:

  Affinity Purified

• Modification:

  Unmodified

• Molecular Weight:

  Calculated MW: 61 kDa; Observed MW: 61 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of Chk2 in HeLa lysates; 293T lysates using Chk2 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4