Raf1 polyclonal antibody

• Background :

  Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.

• Structure Composition :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative :

  RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

• Swiss Prot :

  P04049

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human C-RAF. AA range:251-300

• Conjugation :

  Unconjugated

• Applications :

  ICC/IF, WB, IHC-F, IHC-P, ELISA

• Dilution :

  WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

• Purity :

  Affinity Purified

• Modification :

  Unmodified

• Molecular Weight :

  Calculated MW: 73 kDa; Observed MW: 73 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  IgG

• Applications Notes :

  Western blot analysis of Raf1 in HepG2 lysates using Raf1 antibody.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4