P300 polyclonal antibody

• Background:

  EP300: E1A binding protein p300. This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  EP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300

• Swiss Prot:

  Q09472

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from the Internal region of human p300.

• Conjugation:

  Unconjugated

• Applications:

  ICC/IF, WB, IHC-F, IHC-P, ELISA

• Dilution:

  WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

• Purity:

  Affinity Purified

• Modification:

  Unmodified

• Molecular Weight:

  Calculated MW: 264 kDa; Observed MW: 300 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of p300 in HELA, 293T, 823AV lysates using p300 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4