Phospho-IRS1 (Ser639) polyclonal antibody

• Background:

  May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit .

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  IRS1; Insulin receptor substrate 1; IRS-1

• Swiss Prot:

  P35568

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser639. AA range:605-654

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC-P, ELISA

• Dilution:

  WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

• Purity:

  Affinity Purified

• Modification:

  Phosphorylated

• Molecular Weight:

  Calculated MW: 132 kDa; Observed MW: 150 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of Phospho-IRS1 in various lysates using Phospho-IRS1 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4