P21 polyclonal antibody

• Background:

  The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21

• Swiss Prot:

  P38936

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein corresponding to human p21.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC IF/ICC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF/ICC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 21 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  P21 polyclonal antibody detects endogenous levels of p21 protein.

• Applications Notes:

  Western blot analysis of p21 expression in 293T (A), MCF7 (B), mouse liver (C), Hela (D) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4