DGK-α polyclonal antibody

• Background :

  Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid. DAG and phosphatidic acid are lipids that act as second messengers in signaling cascades. DGK-alpha influences cell activation and secretion of lethal exosomes, which in turn control cell death. DGK-beta is abundant in restricted brain regions such as the caudate putamen and olfactory tubercle. DGK-gamma encodes full-length and truncated transcripts that are present in a range of human tissues, with greatest expression observed in retina. DGK-delta is most abundant in skeletal muscle. DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol and is expressed predominantly in testis. DGK-zeta is most abundant in brain and muscle. DGK-eta is closely related to DGK-delta. DGK-theta is most abundant in the cerebellum and hippocampus.

• CAS Number :

  9007-83-4

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Diacylglycerol kinase alpha; DAG kinase alpha; 80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGK-alpha; DGKA; DAGK; DAGK1

• Swiss Prot :

  P23743

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DGKA (NP_958852.1) .

• Conjugation :

  Unconjugated

• Applications :

  WB

• Dilution :

  WB: 1:100~1:500

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodified

• Molecular Weight :

  ~ 80 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  DGK-α polyclonal antibody detects endogenous levels of DGK-α protein.

• Applications Notes :

  Western blot analysis of extracts of various cell lines, using DGKA pAb antibody at 1:500 dilution.

• Host or Source :

  Rabbit