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SPARC polyclonal antibody

CAT: 0384-BS6738-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS6738-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
SPARC; Basement-membrane protein 40; BM-40; Osteonectin; Short name=ON; Secreted protein acidic and rich in cysteine; SPARC; ON
Swiss Prot
P09486
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1) .
Conjugation
Unconjugated
Related Pathways
Contribution of ECM and Cytoskeletal Factors to EMT
Applications
WB IHC-P IF/ICC
Dilution
WB 1:500 - 1:2000 IHC-P 1:50 - 1:100 IF /ICC 1:50 - 1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 35 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
SPARC polyclonal antibody detects endogenous levels of SPARC protein.
Applications Notes
Western blot analysis of extracts of A375 cells, using SPARC antibody at 1:3000 dilution.
Host or Source
Rabbit

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