CD71/TfR polyclonal antibody

• Background:

  Transferrin receptor 1 (CD71, TFRC) is a type II transmembrane receptor and carrier protein responsible for the uptake of cellular iron through receptor-mediated endocytosis. Neutral pH at the cell surface promotes binding of the iron-binding glycoprotein transferrin (Tf) to the CD71 receptor. The receptor-ligand complex enters the cell through receptor-mediated endocytosis and is internalized into an endosome. Relatively lower endosomal pH leads to a change in the local charge environment surrounding the iron-transferrin binding site and results in the release of iron. The receptor-ligand complex is recycled to the cell surface where transferrin dissociates from the CD71 receptor. Ubiquitously expressed transferrin receptor is continuously recycled and undergoes clathrin-mediated endocytosis regardless of ligand binding state. The interaction between receptor and ligand has been studied in detail. The helical domain of CD71 directly interacts with the transferrin C-lobe and induces a conformation change in Tf to facilitate the transport process. Interaction between the receptor CD71 and transferrin is mediated by the membrane protein hemochromatosis (HFE) . HFE binds the α-helical domain of CD71, blocking formation of the CD71-transferrin complex and inhibiting iron uptake. In addition to binding transferrin, CD71 also interacts with H-ferritin at the cell surface and transports this intracellular iron storage protein to cellular endosomes and lysosomes. Additional studies indicate that the transferrin receptor is an evolutionarily conserved receptor for a number of arenaviruses and at least one retrovirus. Aberrant expression of CD71 is seen in a number of cancers, including thyroid carcinomas, lymphomas, and T-lineage leukemias, suggesting a possible therapeutic role for targeted inhibition of the transferrin receptor.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; T9; p90; CD_antigen: CD71; Transferrin receptor protein 1, serum form; sTfR; TFRC

• Swiss Prot:

  P02786

• Reactivity:

  Human

• Immunogen:

  Recombinant protein of human CD71/TfR.

• Conjugation:

  Unconjugated

• Applications:

  WB IP IF

• Dilution:

  WB: 1:2000~1:5000 IF 1:50~1:200 IP 1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 90 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD71/TfR polyclonal antibody detects endogenous levels of CD71/TfR protein.

• Applications Notes:

  Western blot (WB) analysis of CD71/TfR polyclonal antibody at 1:2000 dilution Lane1:A549 whole cell lysate (30ug) Lane2:L02 whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4