CD47 polyclonal antibody

• Background:

  Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity) .Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD47

• Swiss Prot:

  Q08722

• Reactivity:

  Human

• Immunogen:

  Recombinant protein of human CD47.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB: 1:1000~1:2000 IF 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~45 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD47 polyclonal antibody detects endogenous levels of CD47 protein.

• Applications Notes:

  Western blot (WB) analysis of CD47 polyclonal antibody at 1:1000 dilution Lane1:MCF-7 cell membrane lysate (25ug) Lane2:SGC7901 cell membrane lysate (25ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4