Trx2 polyclonal antibody

• Background:

  Thioredoxin 2 (Trx-2) is a small redox protein that is localized to the mitochondria and is essential for cell viability, playing a crucial role in the scavenging of ROS in mitochondria and regulating the mitochondrial apoptosis signaling pathway. Trx reductases (TrxR1 and TrxR2) are ubiquitously expressed flavoproteins that catalyze the NADPH-dependent reduction of Trx as well as several other oxidized cellular components. Mammalian Trx reductases are a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. TrxR1 and TrxR2 are also involved in the prevention of oxidative stress. Inhibition of TrxR activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Thioredoxin, mitochondrial; MTRX; Mt-Trx; Thioredoxin-2; Thioredoxin 2; Thioredoxin2; TXN2; TRX-2

• Swiss Prot:

  Q99757

• Reactivity:

  Human, Mouse, Rat, Bovine

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Trx2.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 13 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Trx2 polyclonal antibody detects endogenous levels of Trx2 protein.

• Applications Notes:

  Western blot analysis of Trx2 expression in HEK293T (A), Hela (B), mouse liver (C), rat lung (D), rat liver (E) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4