GFAP polyclonal antibody

• Background :

  This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

• Structure Composition :

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  ALXDRD; GFAP

• Swiss Prot :

  P14136

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A synthetic peptide of human GFAP (NP_002046.1) .

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC, IF/ICC

• Dilution :

  WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodified

• Molecular Weight :

  ~ 50 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  Polyclonal Antibodies

• Applications Notes :

  Western blot (WB) analysis of GFAP pAb at 1:1000 dilution Lane1:U-87MG whole cell lysate (30ug) Lane2:SHSY5Y whole cell lysate (30ug) Lane3:EC9706 whole cell lysate (30ug) Lane4:3T3-L1 whole cell lysate (30ug) Lane5:C6 whole cell lysate (30ug)

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4