GFAP polyclonal antibody

• Background:

  This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  ALXDRD; GFAP

• Swiss Prot:

  P14136

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide of human GFAP (NP_002046.1) .

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, IF/ICC

• Dilution:

  WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 50 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Polyclonal Antibodies

• Applications Notes:

  Western blot (WB) analysis of GFAP pAb at 1:1000 dilution Lane1:U-87MG whole cell lysate (30ug) Lane2:SHSY5Y whole cell lysate (30ug) Lane3:EC9706 whole cell lysate (30ug) Lane4:3T3-L1 whole cell lysate (30ug) Lane5:C6 whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4