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Cleaved Caspase-9 P37 polyclonal antibody

CAT: 0384-BS80387-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS80387-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
CASP9; APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56; caspase-9; casp9; Caspase 9
Swiss Prot
P55211
Reactivity
Human, Mouse, Rat
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 9 p37.
Conjugation
Unconjugated
Applications
WB, IHC, IF
Dilution
WB,1:500 - 1:1000|IHC,1:100 - 1:200|IF,1:100 - 1:500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Cleaved
Molecular Weight
~ 37 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cleaved Caspase-9 P37 polyclonal antibody detects endogenous levels of Cleaved Caspase-9 P37 protein.
Applications Notes
Western blot analysis of Caspase 9 p37 expression in A549 (A), HEK293T (B) whole cell lysates.
Host or Source
Rabbit

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