FOSL2 polyclonal antibody

• Background:

  The v-Fos oncogene was initially identified as the transforming gene of two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. The cellular homolog, c-Fos, encodes a nuclear phosphoprotein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers which bind to specific DNA TPA response elements (TREs), c-Fos proteins are only active as heterodimers with members of the Jun gene family. Murine Fos B encodes a nuclear protein of 338 amino acids which has 70% homology with c-Fos, exhibits similar kinetics of expression as c-Fos and forms heterodimers with both c-Jun and Jun B, which bind to TRE DNA response elements. Functional homologs of c-Fos and Fos B include Fra-1 and Fra-2 genes.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Fos-related antigen 2; FRA-2; FOSL2; FRA2

• Swiss Prot:

  P15408

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human FOSL2.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC IF/ICC

• Dilution:

  WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF/ICC 1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 31-35 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  FOSL2 polyclonal antibody detects endogenous levels of FOSL2 protein.

• Applications Notes:

  Western blot analysis of FOSL2 expression in HEK293T (A), Hela (B), H446 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4