CD22 polyclonal antibody

• Background:

  CD22 (also known as siglec-2) is a member of the sialic acid-binding immunoglobulin-type lectin (Siglec) family of immunomodulatory receptors. CD22 can bind to its ligand α 2,6-linked sialic acid on different cells (trans interaction) as well as on the same cells (cis interaction) . CD22 is predominantly expressed on B cells and functions as an inhibitory co-receptor for the B cell receptor (BCR) . After BCR ligation, the tyrosine kinase Lyn is activated and phosphorylates two distal of the four ITIM motifs in the intracellular carboxy-terminal region of CD22, which then recruit tyrosine phosphatases, including SHP-1, to the plasma membrane, and in turn, they get tyrosine-phosphorylated and activated to damp the signaling pathways initiated by BCR ligation. CD22 has been actively pursued as a therapeutic target for autoimmune diseases. Due to almost exclusive expression on B cells, it is also actively pursued as a therapeutic target for multiple B cell malignancies.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD22; SIGLEC2

• Swiss Prot:

  P20273

• Reactivity:

  Human

• Immunogen:

  Recombinant protein of human CD22.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB: 1:2000~1:5000 IF 1:100 - 1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 120 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD22 polyclonal antibody detects endogenous levels of CD22 protein.

• Applications Notes:

  Western blot (WB) analysis of CD22 polyclonal antibody at 1:2000 dilution Lane1:Myla2059 whole cell lysate (30ug) Lane2:HuT78 whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4