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SMAD2/SMAD3 polyclonal antibody

CAT: 0384-BS80508-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS80508-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3
Swiss Prot
P84022/Q15796
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SMAD2/SMAD3 (NP_005892.1) .
Conjugation
Unconjugated
Applications
WB, IHC, IF/ICC
Dilution
WB:1:500 - 1:1000 IHC:1:50 - 1:200 IF/ICC:1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
SMAD2/SMAD3 polyclonal antibody detects endogenous levels of SMAD2/SMAD3 protein.
Applications Notes
Western blot analysis of extracts of various cell lines, using SMAD2/SMAD3 antibody at 1:700 dilution.
Host or Source
Rabbit

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