EGFP polyclonal antibody

CAT: 0384-BS65530-01Size: 1 EachDry Ice: NoHazardous: No
CAT#:0384-BS65530-01Size:1 Each
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Background
Enhanced green fluorescence protein (EGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm) . EGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP fluorescence is stable under fixation conditions and suitable for a variety of applications. EGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize EGFP-tagged proteins within living cells without the need for chemical staining. Other applications of EGFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. EGFP technnology has considerably contributed to a greater understanding of cellular physiology. Description: EGFP produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 239 amino acids and having a molecular mass of 27kDa. EGFP is purified by proprietary chromatographic techniques
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Enhanced Green Fluorecent protein; rEGFP; Recombinant EGFP; Enhanced Green Fluorecent protein
Swiss Prot
P42212
Reactivity
Recombinant EGFP
Immunogen
Recombinant EGFP protin.
Conjugation
Unconjugated
Applications
WB IP
Dilution
WB: 1:1000~1:5000 IP: 1:100~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
EGFP polyclonal Antibody detects endogenous levels of EGFP protein.
Applications Notes
Anti-EGFP at 1/1000 ~ 1/5000 dilution
Host or Source
Rabbit

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