MCM2 mouse monoclonal antibody

• Background:

  The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.

• Structure Composition:

  PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

• Product Name Alternative:

  BM28; CCNL1; cdc19; CDCL1; D3S3194; MITOTIN

• Swiss Prot:

  OTI8A11

• Reactivity:

  Human

• Immunogen:

  Full length human recombinant protein of human MCM2 (NP_004517) produced in HEK293T cell.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC

• Dilution:

  WB 1:500, IHC 1:150

• Purity:

  Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

• Modification:

  Unmodified

• Molecular Weight:

  ~ 120 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  MCM2 mouse monoclonal antibody detects endogenous levels of MCM2 protein.

• Applications Notes:

  Western blot (WB) analysis of MCM2 mouse monoclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate (30ug) Lane2:EC9706 whole cell lysate (30ug) Lane3:SHSY5Y whole cell lysate (30ug)

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4