MAP1LC3B (D23) polyclonal antibody

• Background:

  MAP1LC3B, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3β , the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β , followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3; LC3-I; LC3-II; LC3I; LC3II

• Swiss Prot:

  Q9GZQ8

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant full length protein of human MAP1LC3B.

• Conjugation:

  Unconjugated

• Related Pathways:

  Phosphoinosotode Signaling, Autophagy Signaling

• Applications:

  WB IP

• Dilution:

  WB: 1:1000~1:2000 IP: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 16-18 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  MAP1LC3B (D23) polyclonal antibody detects endogenous levels of LC3-I and LC3-II protein.

• Applications Notes:

  Western blot (WB) analysis of MAP1LC3B (D23) at 1:2000 dilution Lane1:Hela whole cell lysate (30ug) Lane2:U-87MG whole cell lysate (30ug) Lane3:HepG2 whole cell lysate (30ug) Lane4:3T3-L1 whole cell lysate (30ug) Lane5:PC12 whole cell lysate (30ug) Lane6:The Brain tissue lysate of Mouse (30ug) Lane7:The Brain tissue lysate of Rat (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4