Akt2 polyclonal antibody

• Background:

  This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE) . It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  AKT2; HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA

• Swiss Prot:

  P31751

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AKT2.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, IF

• Dilution:

  WB,1:500 - 1:1000|IHC,1:100 - 1:200|IF/ICC,1:100 - 1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 60 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Polyclonal Antibodies

• Applications Notes:

  Western blot analysis of AKT2 expression in A549 (A), LO2 (B), AML12 (C), C6 (D) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4