NEK2 polyclonal antibody

• Background :

  This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  NLK1; RP67; NEK2A; HsPK21; PPP1R111; NEK2

• Swiss Prot :

  P51955

• Reactivity :

  Human, Rat

• Immunogen :

  A synthetic peptide corresponding to a sequence within amino acids 345-445 of human NEK2 (NP_002488.1) .

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC-P

• Dilution :

  WB 1:500 - 1:2000 IHC-P 1:50 - 1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodified

• Molecular Weight :

  ~ 52 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  NEK2 polyclonal antibody detects endogenous levels of NEK2 protein.

• Applications Notes :

  Western blot analysis of lysates from HeLa cells, using NEK2 pAb at 1:1000 dilution.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4