CLIC1 polyclonal antibody

• Background:

  Chloride intracellular channel 1 (CLIC1), also referred to as NCC27, is a member of the highly conserved family of chloride ion channels that function in both soluble and integral membrane forms. CLIC1 is a monomeric protein that contains a redox-active site similar to glutaredoxin; it functions as a anionselective channel. CLIC1 forms a dimer when oxidized and is then able to form chloride ion channels in bilayers and vesicles, whereas a reducing environment prevents this from occurring. Insulin concentration also plays a role in CLIC1 regulation, and the hormone may cause a subnuclear relocalization of CLIC1. CLIC1 is associated with macrophage activation; a downregulation of CLIC1 function prevents TNFα release induced by β-Amyloid protein (A-β) stimulation. This suggests a role for CLIC1 in several neurodegenerative processes, such as Alzheimer’s disease, a syndrome characterized by an accumulation of A-β.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Chloride intracellular channel protein 1; Chloride channel ABP; Nuclear chloride ion channel 27; NCC27; Regulatory nuclear chloride ion channel protein; hRNCC; CLIC1; G6; NCC27

• Swiss Prot:

  O00299

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant Protein corresponding to a sequence within amino acids 42-241 of human CLIC1 (NP_001279.2) .

• Conjugation:

  Unconjugated

• Applications:

  WB IF/ICC IP

• Dilution:

  WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 IP 1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 27 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CLIC1 polyclonal antibody detects endogenous levels of CLIC1 protein.

• Applications Notes:

  Western blot analysis of lysates from MCF7 cells, using CLIC1 pAb at 1:1000 dilution.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4