Products for Research Use Only
RegisterLogin

ATG14 polyclonal antibody

CAT: 0384-BS8969-01Size: 50 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0384-BS8969-01Size:50 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Atg14 (ATG14 autophagy related 14 homolog), also known as BARKOR (beclin 1-associated autophagy-related key regulator) or ATG14L, is a 492 amino acid protein that contains 3 coiled-coil domains in its N-terminal region. Conserved in chimpanzee, canine, bovine, mouse, rat, chicken, zebrafish, fruit fly and mosquito, Atg14 exhibits highest expression in brain and lowest expression in kidney and ovary. Atg14 localizes to isolation membranes of forming autophagosomes, cytoplasm, endoplasmic reticulum and an uncharacterized punctate structure. Necessary for both basal and inducible autophagy, Atg14 plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Atg14 promotes BECN1 translocation from the trans-Golgi network to autophagosomes, and forms a complex with BECN1, PI 3-kinase p100 and p150. Atg14 does not form complexes with KIAA0226/Rubicon or UVRAG, which forms a mutually exclusive complex with BECN1 through direct competition with Atg14.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Beclin 1-associated autophagy-related key regulator; Barkor; Autophagy-related protein 14-like protein; Atg14L; ATG14; KIAA0831
Swiss Prot
Q6ZNE5
Reactivity
Human, Mouse, Rat
Immunogen
Recombination protein, corresponding to amino acids 152-406 of Human ATG14.
Conjugation
Unconjugated
Related Pathways
Autophagy Signaling
Applications
WB IHC
Dilution
WB: 1:500~1:2000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 65 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ATG14 polyclonal antibody detects endogenous levels of ATG14 protein.
Applications Notes
Western blot (WB) analysis of ATG14 pAb at 1:1000 dilution Lane1:H1792 whole cell lysate (20ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug)
Host or Source
Rabbit

Related Products

CatalogName
PPL00331-2aPEGFP-C3-ATG14
PPL00331-1aPET28a-ATG14 (152-406aa)
BS76399-01ATG12 polyclonal antibody
BZ16801-01ATG10 polyclonal antibody
BS90103Atg12 polyclonal antibody
BS70800-01ATG10 polyclonal antibody
BS6045-01ATG13 polyclonal antibody
AP0784-01ATG10 (P220) polyclonal antibody
AP0758-01ATG12 (S129) polyclonal antibody
AP0759-01ATG12 (A98) polyclonal antibody

Alternative Products

CatalogName
ANT-10349-50ugATG14 Polyclonal Antibody
APR15079GPolyclonal ATG14 Antibody
MBS9442130-01ATG14 Polyclonal Conjugated Antibody
NBP2-36445-0.025mgRabbit Polyclonal ATG14 Antibody
GTR17880440ATG14 Rabbit Polyclonal Antibody
TMAB-02911-01Anti-ATG14 Polyclonal Antibody
APR15080GPolyclonal ATG14 Antibody (Internal)
MBS8508779-01ATG14 Antibody (Rabbit Polyclonal)

Popular Products