DDX20 polyclonal antibody

CAT: 0384-BS61793-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS61793-01Size:50 µL
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Background
Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of motor neurons in the spinal cord. SMA is caused by deletion or loss-of-function mutations in the SMN (survival of motor neuron) gene. Gemin3, also known as DP103, DDX20, DEAD-box protein DP130 and DEAD/H box 20, is a protein product of human chromosome 1p13.2. It associates directly with SMN and is a part of the SMN complex containing Gemin2, Gemin4, Gemin5 and Gemin6, as well as several spliceosomal snRNP proteins. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing of the nucleus. It is found in both the cytoplasm and the nucleus. The nuclear form is concentrated in subnuclear bodies called gems (for Gemini of the coiled bodies) . Gemin3 also interacts with SmB, SmD2 and SmD3. It contains the conserved motif Asp-Glu-Ala-Asp (DEAD) characteristic of DEAD-box proteins. Gemin3 is a putative RNA helicase and shows ATPase activity. It is expressed in B and T cell neuroblastoma-derived cell lines, malignant melanoma tumor, normal testis and is expressed in low levels in colon, skeletal muscle, liver, kidney and lung.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Probable ATP-dependent RNA helicase DDX20; Component of gems 3; DEAD box protein 20; DEAD box protein DP 103; Gemin-3; DDX20; GEMIN3
Swiss Prot
Q9UHI6
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 270-320 of Human DDX20.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 110 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
DDX20 pAb detects endogenous levels of DDX20 protein.
Applications Notes
Western blot (WB) analysis of DDX20 pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:HCC827 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug)
Host or Source
Rabbit

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