MRPL13 polyclonal antibody

• Background:

  Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  39S ribosomal protein L13, mitochondrial; L13mt; MRP-L13

• Swiss Prot:

  Q9BYD1

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 125-169 of Human MRPL13.

• Conjugation:

  Unconjugated

• Applications:

  WB IP

• Dilution:

  WB: 1:500~1:1000 IP: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 20 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  MRPL13 polyclonal antibody detects endogenous levels of MRPL13 protein.

• Applications Notes:

  Western blot (WB) analysis of MRPL13 polyclonal antibody at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4