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Vimentin (phospho-S56) polyclonal antibody

CAT: 0384-BS4539-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS4539-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Phosphorylation of Vimentin induces disassembly of Vimentin intermediate filaments in vivo and in vitro. Binding of 14-3-3 depends on Vimentin phosphorylation and requires the phosphopeptide binding domain of 14-3-3, which is an amino terminal head domain consisting of amino acids 1-96. Phosphorylated Vimentin sequesters 14-3-3 and limits its availability to other target proteins, which can affect intracellular signaling processes that require 14-3-3. The amino-terminal domain of Vimentin is the target site for several protein kinases, including Rho kinase and PKC. Ser 38 and Ser 71 of Vimentin are the major sites of phosphorylation by Rho kinase. The disruption of subcellular compartmentalization of interphase cells leads to PKC-mediated phosphorylation of Vimentin. Thus, targeting of activated PKC, coupled with the reorganization of intracellular membranes, which contain phospholipids essential for activation, leads to the mitosis-specific phosphorylation of Vimentin.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Vimentin; VIM
Swiss Prot
P08670
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Vimentint around the phosphorylation site of Serine 56.
Conjugation
Unconjugated
Related Pathways
Neuronal and Glial Cell Marker Atlas, Contribution of ECM and Cytoskeletal Factors to EMT
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 57 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Vimentin (S56) polyclonal antibody detects endogenous levels of Vimentin protein when phosphorylated at Ser56.
Applications Notes
Western blot (WB) analysis of p-Vimentin (S56) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40µg) Lane2:A375 whole cell lysate (40µg) Lane3:C6 whole cell lysate (40µg) Lane4:The testis tissue lysate of Mouse (40µg) Lane5:Hela whole cell lysate (40µg)
Host or Source
Rabbit

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