DPF2 (E174) polyclonal antibody

• Background:

  DPF2 (D4, zinc and double PHD fingers family 2), also known as REQ (Requiem), UBID4 or ubi-d4, is a 391 amino acid protein that is a member of the D4 domain family. DPF2, a ubiquitously expressed protein, localizes to the nucleus and contains one C2H2- and two PHD-type zinc finger motifs. DPF2 may function as a transcription factor that is necessary for apoptosis and may also play a role in the development and maturation of lymphoid cells. It is thought that, during apoptosis, DPF2 activity is inhibited by LRF (Leukemia/lymphoma-related factor), which is upregulated by integrin. This suggests that DPF2 may be a potential target for future cancer therapies that induce apoptosis in leukemia cells. Alternative splicing of this gene generates multiple isoforms lacking certain domain.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Apoptosis response zinc finger protein; BAF45D; BRG1-associated factor 45D; D4; D4 zinc and double PHD fingers family 2; double PHD fingers family 2; DPF 2; DPF2; MGC10180; Protein requiem; REQ; REQU; Requiem; Requiem apoptosis response zinc finger; UBI D4; ubi-d4; UBID 4; UBID4; zinc and double PHD fingers family 2; Zinc finger protein ubi d4

• Swiss Prot:

  Q92785

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 140-188 of Human DPF2.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 44 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  DPF2 (E174) polyclonal antibody detects endogenous levels of DPF2 protein.

• Applications Notes:

  Western blot (WB) analysis of DPF2 (E174) polyclonal antibody at 1:1000 dilution Lane1:THP-1 whole cell lysate (37ug) Lane2:MCF-7 whole cell lysate (48ug) Lane3:RAW264.7 whole cell lysate (57ug) Lane4:NIH3T3 whole cell lysate (48ug) Lane5:H9C2 whole cell lysate (37ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4