DPF2 (E174) polyclonal antibody

CAT: 0384-BS2668-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS2668-01Size:50 µL
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Background
DPF2 (D4, zinc and double PHD fingers family 2), also known as REQ (Requiem), UBID4 or ubi-d4, is a 391 amino acid protein that is a member of the D4 domain family. DPF2, a ubiquitously expressed protein, localizes to the nucleus and contains one C2H2- and two PHD-type zinc finger motifs. DPF2 may function as a transcription factor that is necessary for apoptosis and may also play a role in the development and maturation of lymphoid cells. It is thought that, during apoptosis, DPF2 activity is inhibited by LRF (Leukemia/lymphoma-related factor), which is upregulated by integrin. This suggests that DPF2 may be a potential target for future cancer therapies that induce apoptosis in leukemia cells. Alternative splicing of this gene generates multiple isoforms lacking certain domain.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Apoptosis response zinc finger protein; BAF45D; BRG1-associated factor 45D; D4; D4 zinc and double PHD fingers family 2; double PHD fingers family 2; DPF 2; DPF2; MGC10180; Protein requiem; REQ; REQU; Requiem; Requiem apoptosis response zinc finger; UBI D4; ubi-d4; UBID 4; UBID4; zinc and double PHD fingers family 2; Zinc finger protein ubi d4
Swiss Prot
Q92785
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 140-188 of Human DPF2.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 44 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
DPF2 (E174) polyclonal antibody detects endogenous levels of DPF2 protein.
Applications Notes
Western blot (WB) analysis of DPF2 (E174) polyclonal antibody at 1:1000 dilution Lane1:THP-1 whole cell lysate (37ug) Lane2:MCF-7 whole cell lysate (48ug) Lane3:RAW264.7 whole cell lysate (57ug) Lane4:NIH3T3 whole cell lysate (48ug) Lane5:H9C2 whole cell lysate (37ug)
Host or Source
Rabbit

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