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Adducin β (E497) polyclonal antibody

CAT: 0384-BS2523-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS2523-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Adducins are a family of cytoskeleton proteins encoded by three genes (α, β and γ) . Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin α is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin α and Adducin γ is ubiquitous in contrast to the restricted expression of Adducin β. Adducin β is expressed at high levels in brain and hematopoietic tissues, such as bone marrow in humans, and spleen in mice.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Beta-adducin; Erythrocyte adducin subunit beta; ADD2; ADDB; β-Adducin; Adducin1; Adducin-1; β Adducin; Adducin β; Adducin-β
Swiss Prot
P35612
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 470-520 of Human Adducin β.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 110 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Adducin β (E497) polyclonal antibody detects endogenous levels of Adducin β protein.
Applications Notes
Western blot (WB) analysis of Adducin β (E497) pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:The Brain tissue lysate of Mouse (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:K562 whole cell lysate (40ug)
Host or Source
Rabbit

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