Adducin β (E497) polyclonal antibody

• Background:

  Adducins are a family of cytoskeleton proteins encoded by three genes (α, β and γ) . Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin α is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin α and Adducin γ is ubiquitous in contrast to the restricted expression of Adducin β. Adducin β is expressed at high levels in brain and hematopoietic tissues, such as bone marrow in humans, and spleen in mice.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Beta-adducin; Erythrocyte adducin subunit beta; ADD2; ADDB; β-Adducin; Adducin1; Adducin-1; β Adducin; Adducin β; Adducin-β

• Swiss Prot:

  P35612

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 470-520 of Human Adducin β.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC IF

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 110 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Adducin β (E497) polyclonal antibody detects endogenous levels of Adducin β protein.

• Applications Notes:

  Western blot (WB) analysis of Adducin β (E497) pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:The Brain tissue lysate of Mouse (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:K562 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4