ABL1 (E883) polyclonal antibody

• Background:

  The Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV) . The major translational product of c-Abl has been identified as a protein with tyrosine kinase activity and an SH2 domain. The Abl oncogene is implicated in several human leukemias including 90-95% of chronic myelocytic leukemia (CML), 20-25% of adult acute lymphoblastic leukemia (ALL) and 2-5% of pediatric ALL. In these leukemias the c-Abl proto-oncogene undergoes a (9;22) chromosomal translocation producing the Philadelphia (Ph1) chromosome. The molecular consequence of this translocation is the generation of a chimeric Bcr/c-Abl mRNA encoding activated Abl protein-tyrosine kinase. The Bcr gene has been shown to encode a GTPase-activating protein (GAP) specific for the Ras-related GTP-binding protein, p21rac.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150; ABL1; ABL; JTK7

• Swiss Prot:

  P00519

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 851-900 of Human ABL1.

• Conjugation:

  Unconjugated

• Related Pathways:

  SAPK JNK Signaling Cascades

• Applications:

  WB IHC IF

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 150 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  ABL1 (E883) polyclonal antibody detects endogenous levels of ABL1 protein.

• Applications Notes:

  Western blot (WB) analysis of ABL1 (E883) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4