AIM2 (A78) polyclonal antibody

• Background:

  Interferon-inducible protein AIM2 (absent in melanoma 2) is a 343 amino acid protein belonging to the HIN-200 family. Induced by IFN-γ, AIM2 is thought to act as a tumor suppressor by repressing NFκB transcriptional activity. Localized to the nucleus, AIM2 contains one DAPIN domain and one HIN-200 domain. The DAPIN domain is composed mostly of α-helixes and is a protein-protein interaction domain capable of binding other DAPIN domains. The HIN-200 domain has been shown to bind directly to DNA, which, along with the binding of another protein ASC, results in the activation of caspase-1. AIM2 is present as a homodimer and is expressed highly in small intestine, testis, peripheral blood leukocytes and spleen. Defects in AIM2 are believed to be a cause of microsatellite unstable colon cancers.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Interferon-inducible protein AIM2; Absent in melanoma 2

• Swiss Prot:

  O14862

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide, corresponding to amino acids 51-100 of Human AIM2.

• Conjugation:

  Unconjugated

• Related Pathways:

  Inflammasonme Signaling

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 39 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  AIM2 (A78) polyclonal antibody detects endogenous levels of AIM2 protein.

• Applications Notes:

  Western blot (WB) analysis of AIM2 (A78) pAb at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4