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HDAC 9 (P1047) polyclonal antibody

CAT: 0384-BS1168-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS1168-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1–9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Histone deacetylase 9; Short name=HD9; Histone deacetylase 7B; Short name=HD7; Short name=HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR
Swiss Prot
Q9UKV0
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to the C-terminual of Human HDAC 9.
Conjugation
Unconjugated
Related Pathways
Cell Cycle Control G1 S Checkpoint
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 120 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
HDAC9 (P1047) polyclonal antibody detects endogenous levels of HDAC9 protein.
Applications Notes
Western blot (WB) analysis of HDAC 9 (P1047) polyclonal antibody at 1:500 dilution Lane1:Rat Brain tissue lysate Lane2:Mouse Brain tissue lysate Lane3:SGC7901 cell lysate Lane3:HEK293T cell lysate Lane3:MCF-7 cell lysate
Host or Source
Rabbit

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