AChE polyclonal antibody

• Background :

  Acetylcholinesterase (AChE) hydrolyzes acetylcholine at synaptic junctions. Alternative mRNA splicing gives rise to three forms of AChE. The T form, also known as the asymmetric form, is soluble and is present in synapses. The H form is also known as the globular form and is present on the outer surfaces of cell membranes. The R form is not known to be a functional species. AChE globular form subunits are GPI-anchored to cell membranes and asymmetric subunits are anchored to basal lamina components by a collagen tail. The catalytic sununits of AChE are oligomers composed of disulfide-linked homodimers. The loss of AChE from cholinergic and noncholinergic neurons in the brain is seen in patients with Alzheimer’s disease. However, AChE activity is increased around amyloid plaques, which may be due to a disturbance in calcium homeostasis involving the opening of L-type voltage-dependent calcium channels.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Acetylcholinesterase; AChE; ACHE; ACES

• Swiss Prot :

  P22303

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide corresponding to residues in Human AChE.

• Conjugation :

  Unconjugated

• Applications :

  WB IHC

• Dilution :

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 117% (by SDS-PAGE) .

• Modification :

  Unmodified

• Molecular Weight :

  ~ 70-85 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  ACHE polyclonal antibody detects endogenous levels of ACHE protein.

• Applications Notes :

  Western blot (WB) analysis of AChE pAb at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:The Blood tissue lysate of Mouse (40ug) Lane4:The Blood tissue lysate of Rat (40ug)

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4