LC3B polyclonal antibody

• Background:

  The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B; LC3A/LC3B

• Swiss Prot:

  Q9GZQ8

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human LC3B (NP_073729.1) .

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, IF/ICC

• Dilution:

  WB,1:500 - 1:2000|IHC,1:200 - 1:500|IF/ICC,1:100 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  14KDa/16KDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  LC3B polyclonal antibody detects endogenous levels of LC3B protein.

• Applications Notes:

  Western blot analysis of LC3B expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4